Composite

Part:BBa_K3396006:Design

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)


GFPnano-Coh2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 35


Design Notes

Keeping the binding of TRIM21 and GFPnano, we make the GFPnano tagged with Coh2, which could connect with DocS spontaneously.

Source

The sequence of GFP-nano we use came from a patent and Coh2 is originally expressed in C. thermocellum scaffoldin

References